Impact of in Vitro Heat Shock (42.50c) on Prostaglandins, Ionic and Metabolic Contents in Sheep Endometrial Epithelial Cells
نویسندگان
چکیده
Higher environmental temperature is one of the major factors responsible for reduced fertility in farm animals. Heat stress causes a great suppression in endometrial function [1] besides compromised follicular growth [2], embryonic function [3] and oocyte developmental potential [4]. Heat stress also increases the production of PGF2α in the endometrium, leading to the early regression of CL or the death of embryos [5]. Heat stress-induced hyperthermia has been found to decrease the pregnancy rate in cattle during the summer in regions associated with elevated ambient temperatures [6]. Prostaglandins (PGs) produced by endometrium serves as a crucial mediators in maternal recognition of pregnancy, implantation and parturition [7,8]. The endometrial epithelial cells preferentially produce PGF2α whereas stromal cells produce mainly PGE2. PGF2α acts as the luteolytic agent to control the estrous cycle in ruminants. Endometrial secretion of PGF2α by pregnant uterus has been found to increase in response to heat stress and decrease the embryonic survivality by altering the signals required for maintenance of corpus luteum function during early pregnancy. Increased PGF2α synthetic capacity of endometrium exposed to heat stress may be due to heat-induced alterations in endometrial cellular membranes resulting in increased mobilization of substrate for prostaglandin biosynthesis. Similar increases in uterine PGF2α secretion in response to heat stress in vitro by endometrial expiants from cows at Day 17 of pregnancy have been reported [5]. No reports are available on impact of in vitro heat stress on endometrial epithelial prostaglandins, ionic and metabolic contents in sheep. The present study was undertaken to investigate the effect of heat shock on prostaglandins, ionic and metabolic contents of sheep endometrial epithelial cells in vitro.
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